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. 2019 Apr 2;16:419–433. doi: 10.1016/j.omtn.2019.03.007

Figure 3.

Figure 3

miR-16-1-3p, miR-4473, and miR-520f-3p Partially Prevent the Activation of STAT3 upon hy-IL-6 Stimulation by Targeting gp130 mRNA, Reducing the Expression of a hy-IL-6 Target Gene in PH5CH8 Cells

Cells were left untreated, reverse transfected with NCM1 or miR-16-1-3p, miR-4473, and miR-520f-3p mimics (20 nM), and were stimulated with hy-IL-6 (20 ng/mL). Single mimics as well as combinations were tested and compared to the respective NCM1 control (in black). For the double or triple combinations, 40 or 60 nM NCM1 was used as the respective control. (A) Normalized mRNA expression level of gp130. (B) Representative western blot analysis of both the activated and total forms of STAT3 and of the Vinculin as loading control. (C) Normalized mRNA expression level of Lipopolysaccharide-binding protein (LBP). The mRNA levels were monitored upon mimic transfection and hy-IL-6 stimulation (depicted with dashed lines). Error bars represent the SD of at least 3 biological replicates. Bottom indicates the applied treatments per lane.