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. 2019 Apr 3;20(7):1654. doi: 10.3390/ijms20071654

Figure 2.

Figure 2

Dose response study of Akt activation by rat C-peptide in L6 cells. Cells were stimulated with rat C-peptide for 5 min in DMEM. Pertussis toxin (PTX) denotes the effect of co-incubation with 100 ng/mL Pertussis toxin. Phosphorylation of Akt was determined by the Western blot (A) using specific anti-phospho-Akt antibody. DFBS (2%) was used as a positive control. As a loading control, membranes were reprobed with antibody against total Akt; (B) quantification by densitometry of data from three such experiments: mean ± SEM * p < 0.05 versus the unstimulated control.