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. 2019 Apr 11;24(7):1432. doi: 10.3390/molecules24071432

Figure 3.

Figure 3

Figure 3

Co-localization of eCB-binding receptors with N-acetylglucosamine (GlcNAc). Representative triple immunofluorescence staining of GlcNAc (green), eCB-binding receptors (red), and DAPI (blue) in SH-SY5Y cells. SH-SY5Y cells were incubated with a biotinylated lectin (WGA) to visualize total GlcNAc, and with specific antibodies [anti-CB1 (A), anti-CB2 (B), anti-GPR55 (C), and anti-TRPV1 (D)] for eCB-binding receptors; then with Alexa Fluor 488-labelled streptavidine for GlcNAc residues, with anti-rabbit IgG labeled with Alexa Fluor 595 for eCB-binding receptors and with DAPI for DNA. Scale bars = 50 μm.