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. 2019 Apr 2;24(7):1290. doi: 10.3390/molecules24071290

Figure 3.

Figure 3

Effects of apigenin on the NF-κB signaling pathway in HMC-1 cells. Cells were pre-treated with apigenin at indicated concentrations for 1 h prior to PI stimulation for 30 min. Western blot analysis was performed to evaluate the expressions of molecules of the NF-κB signaling pathway in the cells. The bands were quantitated using Image J analysis software tool. The data presented are the means ± SD of three independent experiments. Bars with different small case letters indicated statistically significant difference at p < 0.05. PI, phorbol-12-myristate 13-acetate (50 nM) and ionomycin (1 μM).