Figure 5.

Effects of apigenin on compound 48/80-induced infiltration of mast cell and IL-31 expression in mice skin. Five mice each were placed into four groups: Compound 48/80 negative control; compound 48/80 positive control; compound 48/80 with apigenin 75 mg/kg treatment; and compound 48/80 with apigenin 150 mg/kg treatment. Compound 48/80 (50 μg/site, 0.1 mL each) was injected in the dorsal dermis of the mice neck 60 min after administration of 2% gelatin or apigenin at indicated concentrations. Skin tissue sections were stained with hematoxylin/eosin stains showing infiltration of polymorphonuclear leukocytes (A); toluidine blue showing mast cell infiltration into the skin (B); immunohistochemistry was done to demonstrate tryptase (C) and IL-31 (D) in the mice skin.