Table 2.
Regression equation, limits of detection, limits of quantification, precision, repeatability, stability, and recovery of 30 investigated compounds.
| No. | Compounds | Regression Equation | r2 | Liner Range (ng/mL) | LODs (ng/mL) | LOQs (ng/mL) | Precision (RSD, %) | Repeatability (RSD, %) (n = 6) | Stability (RSD, %) (n = 6) | Recovery (%) (n = 3) | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Intra-Day (n = 6) | Inter-Day (n = 3) | Low | Medium | High | ||||||||||||
| Mean | RSD | Mean | RSD | Mean | RSD | |||||||||||
| 1 | Quinic acid | Y = 1330X + 76100 | 1.0000 | 133 – 13300 | 2.66 | 6.57 | 2.40 | 2.63 | 3.5 | 1.1 | 99.55 | 0.22 | 99.65 | 0.36 | 99.83 | 0.05 |
| 2 | d(−)-Tartaric acid | Y = 1240X + 1190 | 0.9995 | 3.75 – 24.9 | 0.49 | 1.62 | 3.09 | 1.60 | 2.1 | 2.3 | 95.62 | 2.1 | 98.07 | 0.57 | 97.34 | 0.74 |
| 3 | l-(−)-Malic acid | Y = 2040X + 149000 | 0.9991 | 148 – 29500 | 2.95 | 12.42 | 1.79 | 1.45 | 3.2 | 1.2 | 99.92 | 0.06 | 99.93 | 0.03 | 99.96 | 0.06 |
| 4 | Protocatechuic acid | Y = 162X + 3100 | 0.9997 | 255 – 12800 | 1.96 | 9.03 | 2.31 | 1.27 | 2.5 | 3.3 | 99.98 | 0.01 | 99.96 | 0.02 | 99.93 | 0.06 |
| 5 | Schisandrin | Y = 21.3X + 1550 | 0.9991 | 11.6 – 9320 | 1.36 | 7.84 | 3.06 | 1.86 | 0.7 | 3.1 | 99.36 | 0.3 | 99.8 | 0.1 | 99.63 | 0.06 |
| 6 | Gomisin D | Y = 3770X + 34900 | 0.9992 | 10.1 – 506 | 0.84 | 6.61 | 1.11 | 0.81 | 1.5 | 1.2 | 96.63 | 2.36 | 96.96 | 1.87 | 96.33 | 4.12 |
| 7 | Gomisin J | Y = 739X + 7100 | 0.9993 | 23.3 – 931 | 1.53 | 6.07 | 2.03 | 1.26 | 3.0 | 3.0 | 98.63 | 0.62 | 98.59 | 0.54 | 97.7 | 0.45 |
| 8 | Schisandrol B | Y = 4140X + 260000 | 0.9997 | 107 – 5350 | 1.92 | 8.15 | 2.56 | 0.31 | 2.4 | 0.8 | 99.05 | 0.38 | 98.85 | 0.18 | 99.13 | 0.7 |
| 9 | Angeloylgomisin H | Y = 25800X + 623000 | 0.9991 | 5.65 – 2260 | 1.36 | 5.38 | 2.45 | 0.15 | 1.6 | 3.1 | 99.84 | 0.22 | 99.24 | 0.74 | 99.68 | 0.32 |
| 10 | Schizantherin B | Y = 240X + 3640 | 0.9998 | 55.2 – 2210 | 2.29 | 12.65 | 1.00 | 0.54 | 1.3 | 2.3 | 99.02 | 0.34 | 98.98 | 0.73 | 99.33 | 0.69 |
| 11 | Schisanhenol | Y = 2900X + 10800 | 0.9999 | 19.9 – 1194 | 1.99 | 5.82 | 2.59 | 1.58 | 4.9 | 1.5 | 98.19 | 1.12 | 98.63 | 0.88 | 96.92 | 3.3 |
| 12 | Deoxyschizandrin | Y = 26600X + 217000 | 1.0000 | 24 – 2400 | 2.91 | 11.21 | 0.87 | 1.10 | 1.6 | 1.3 | 97.83 | 0.74 | 98.86 | 0.34 | 99.42 | 0.02 |
| 13 | γ-schisandrin | Y = 15700X + 214000 | 1.0000 | 15.2 – 4560 | 1.79 | 6.92 | 1.91 | 0.43 | 0.5 | 1.1 | 99.63 | 0.3 | 99.75 | 0.09 | 99.69 | 0.13 |
| 14 | Schizandrin C | Y = 6410X + 24300 | 0.9991 | 29.3 – 1470 | 0.52 | 1.38 | 2.25 | 0.66 | 3.8 | 2.5 | 98.57 | 0.41 | 97.64 | 0.61 | 98.24 | 0.61 |
| 15 | Schisantherin | Y = 25X +1860 | 0.9998 | 12 – 7200 | 1.59 | 5.62 | 0.67 | 1.61 | 2.2 | 1.0 | 99.89 | 0.04 | 99.89 | 0.04 | 99.97 | 0.02 |