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. 2019 Mar 27;20(7):1541. doi: 10.3390/ijms20071541

Figure 1.

Figure 1

SPARC enhances NGFβ-dependent ERK activation and neurite outgrowth in PC12 cells. (A) PC12 cells were treated with NGFβ (1 ng/mL) in the presence or absence of SPARC (0.1 or 1 µg/mL) for 10 min. Representative results of Western blots for ERK and its phosphorylation are shown in the upper panel, Results from four independent experiments are summarized in the bottom panel. (B) PC12 cells were treated with or without NGFβ (0 or 1 ng/mL) either in the presence or absence of SPARC (0.1 or 1 µg/mL) for 96 h. Representative results of cells with neurites are shown in the upper panel and results (total neurite length per cell) from three independent experiments are summarized in the bottom. The length of the scale bar in the picture is 50 µm. * and † indicate significant differences (p < 0.05) between no NGFβ treatment (0 ng/mL) vs NGF treated and no SPARC treatment (0 µg/mL) vs SPARC treated, respectively.