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. 2019 Mar 27;20(7):1541. doi: 10.3390/ijms20071541

Figure 3.

Figure 3

Adiponectin suppressed NGFβ-induced neurite outgrowth independently of its receptor activation. (A) Expression of AdipoR1 and AdipoR2 mRNA in the rat skeletal muscle (SM), liver and PC12 cells are shown. (B) PC12 cells were treated with full length adiponectin or globular adiponectin and the amounts of phosphorylated and total AMPK were determined. Representative results and the ratio of phosphorylated and total AMPK are shown (n = 5). (C–E) PC12 cells were treated with unrelated (un), AdipoR1, AdipoR2 and R1 plus R2 siRNA and (C) mRNA expression of AdipoR1 and AdipoR2 are shown. (D) The transfected cells were treated with vehicle (cont.), globular adiponectin (1 µg/mL) and full length adiponectin (1 µg/mL) and the state of AMPK activation are shown (n = 3). (E) The transfected cells were treated with vehicle (cont.), NGFβ (1 ng/mL) or NGFβ plus full length adiponectin (1 µg/mL) and the ratios of the cell with axon are shown (n = 3). The transfected cells treated with vehicle did not induce any neurite (axon) as shown in Figure 2A and the ratio calculated was 0 as in Figure 2B. Thus, bar for control value of each siRNA was not seen. * indicates the statistically significant difference (p < 0.05) from cont. or NGFβ treatment alone.