Table 2.
Linear regression, LOD, LOQ and precision, stability, repeatability, recovery of the analytes.
| No. | Regression Equation | R2 | Linear Range (ng/mL) | LOD | LOQ | Precision (RSD, %) | Stability (RSD, %) | Repeatability (RSD, %) | Recovery | ||
|---|---|---|---|---|---|---|---|---|---|---|---|
| (ng/mL) | (ng/mL) | Intraday | Interday | Mean, % | RSD, % | ||||||
| 1 | y = 2,824,247.9x + 5,954,683.1 | 0.9978 | 2.500–4.167 × 104 | 0.13 | 0.34 | 2.32 | 3.02 | 3.42 | 2.42 | 101.3 | 2.11 |
| 2 | y = 922,961x + 568,615 | 0.9986 | 5.100–5.875 × 103 | 0.58 | 1.7 | 1.32 | 2.42 | 3.43 | 4.23 | 98.0 | 3.01 |
| 3 | y = 1,486,176.5x + 632,070.7 | 0.9968 | 2.700–1.130 × 104 | 0.11 | 0.34 | 0.92 | 2.23 | 4.01 | 3.92 | 103.3 | 3.07 |
| 4 | y = 864,935x + 584,181 | 0.9987 | 2.600–4.300 × 104 | 0.13 | 0.86 | 2.16 | 3.12 | 4.21 | 4.64 | 97.4 | 4.05 |
| 5 | y = 12,340,559.2x + 13,971,574.7 | 0.9971 | 5.300–2.300 × 104 | 0.46 | 2.4 | 2.53 | 3.24 | 3.98 | 3.13 | 104.2 | 3.75 |
| 6 | y = 4,394,306.8x + 3,947,119.7 | 0.9987 | 4.000–2.001 × 104 | 0.39 | 1.5 | 3.04 | 3.52 | 4.06 | 4.14 | 96.4 | 3.13 |
| 7 | y = 327,479x + 18,539 | 0.9988 | 14.00–3.603 × 103 | 1.4 | 6.9 | 2.91 | 3.12 | 3.42 | 4.01 | 97.4 | 3.37 |
| 8 | y = 4,095,340.6x + 4,855,604.6 | 0.9982 | 5.200–4.100 × 104 | 0.84 | 2.9 | 3.43 | 3.32 | 2.94 | 3.54 | 103.2 | 2.95 |
| 9 | y = 439,258x + 879,574 | 0.9939 | 10.10–4.603 × 104 | 0.91 | 2.7 | 1.93 | 2.42 | 2.95 | 3.24 | 102.3 | 2.82 |
| 10 | y = 17,063,239.4x + 149,593.2 | 0.9974 | 0.7000–1.900 × 102 | 0.018 | 0.039 | 2.82 | 3.02 | 3.12 | 3.32 | 99.4 | 3.21 |
| 11 | y = 14,470,625.6x + 1,549,987.5 | 0.9937 | 4.000–2.500 × 103 | 0.68 | 2.5 | 3.03 | 2.94 | 2.84 | 3.23 | 102.4 | 2.94 |
| 12 | y = 7,629,191.3x + 3,135,983.9 | 0.9911 | 2.000–3.600 × 104 | 0.21 | 0.36 | 2.95 | 3.13 | 3.65 | 3.64 | 103.5 | 3.53 |
| 13 | y = 4,083,739.7x + 7,735,801.6 | 0.9921 | 2.500–1.260 × 105 | 0.13 | 0.37 | 3.54 | 3.74 | 3.96 | 3.44 | 96.4 | 2.95 |
| 14 | y = 2,948,202.5x + 1,270,029.0 | 0.9991 | 4.600–1.140 × 105 | 0.46 | 1.1 | 3.05 | 3.54 | 2.96 | 3.04 | 102.4 | 3.05 |
| 15 | y = 7,971,028.9x + 2,483,617.9 | 0.9999 | 6.000–5.101 × 104 | 0.51 | 2.0 | 2.95 | 3.03 | 3.32 | 3.54 | 104.5 | 3.41 |
| 16 | y = 4,223,984.3x + 555,633.9 | 0.9989 | 8.000–3.603 × 104 | 0.83 | 3.6 | 3.03 | 3.21 | 3.33 | 2.63 | 103.6 | 2.94 |
| 17 | y = 4,587,940.9x – 483,834.6 | 0.9997 | 12.10–5.102 × 104 | 0.50 | 1.3 | 3.09 | 3.86 | 4.05 | 3.53 | 96.4 | 3.53 |
| 18 | y = 4,304,705.8 x + 10,445.9 | 0.9996 | 11.00–4.300 × 104 | 0.42 | 1.4 | 4.04 | 3.95 | 4.22 | 3.95 | 102.4 | 3.89 |
| 19 | y = 960,985x + 794,899 | 0.9971 | 8.000–1.020 × 105 | 0.30 | 1.0 | 3.91 | 2.94 | 4.02 | 3.84 | 103.5 | 4.04 |
| 20 | y = 1,828,288.2x – 155,615.5 | 0.9992 | 5.000–4.201 × 104 | 0.53 | 1.4 | 3.53 | 4.02 | 3.95 | 4.23 | 98.3 | 3.94 |
| 21 | y = 2,631,959.6x + 231,832.9 | 0.9998 | 8.100–3.500 × 104 | 0.35 | 1.1 | 3.94 | 3.89 | 3.52 | 3.56 | 104.2 | 4.12 |
| 22 | y = 115,788x + 44,801 | 0.9941 | 70.10–3.702 × 104 | 3.7 | 11 | 3.53 | 2.94 | 3.45 | 4.05 | 102.6 | 3.64 |
| 23 | y = 1,263,773.8x + 124,249.7 | 0.9996 | 30.10–3.502 × 104 | 3.0 | 9.7 | 4.03 | 3.52 | 3.89 | 3.51 | 97.4 | 3.52 |
| 24 | y = 195,275.9x + 46,906.5 | 0.9988 | 1.588×102–2.033×104 | 15 | 41 | 4.21 | 3.81 | 3.52 | 4.02 | 103.5 | 4.01 |
| 25 | y = 1,557,660.4x – 664,389.7 | 0.9974 | 25.00–5.310 × 104 | 5.4 | 12 | 3.85 | 3.53 | 3.90 | 4.13 | 102.4 | 3.89 |
| 26 | y = 550,153x – 170,668 | 0.9978 | 20.20–4.301 × 104 | 0.43 | 1.4 | 3.31 | 2.53 | 2.95 | 3.69 | 101.5 | 3.64 |
| 27 | y = 1,101,798.4x – 441,695.7 | 0.9955 | 31.00–3.370 × 104 | 0.39 | 1.2 | 3.21 | 3.32 | 3.13 | 4.01 | 97.5 | 4.64 |
| 28 | y = 2,056,201.7x – 1,509,290.6 | 0.9932 | 19.00–3.903 × 104 | 0.73 | 2.0 | 3.53 | 2.95 | 3.34 | 2.95 | 103.5 | 2.95 |
| 29 | y = 441,570x + 48,517 | 0.9997 | 80.10–4.500 × 104 | 0.48 | 1.4 | 3.63 | 2.94 | 3.52 | 2.53 | 98.4 | 3.04 |
| 30 | y = 261,545x – 89,819 | 0.9994 | 1.300 × 102–6.700 × 104 | 1.9 | 6.7 | 2.98 | 3.02 | 3.32 | 3.52 | 102.5 | 4.04 |
| 31 | y = 1,501,631.2x – 976,172.6 | 0.9995 | 16.00–6.602 × 104 | 0.67 | 1.7 | 3.02 | 2.94 | 3.12 | 3.14 | 100.4 | 3.53 |
| 32 | y=3,645,684x – 98,187 | 0.9989 | 1.360 × 102–3.502 × 104 | 0.97 | 2.7 | 2.79 | 3.10 | 2.93 | 4.02 | 97.2 | 2.99 |
| 33 | y = 506,624x – 224,402 | 0.9922 | 1.300 × 102–1.781 × 104 | 0.65 | 1.8 | 3.02 | 2.95 | 3.04 | 4.04 | 99.4 | 3.02 |
| 34 | y = 255,220x + 20,740 | 0.9912 | 60.10–1.600 × 103 | 0.64 | 1.6 | 2.94 | 3.04 | 3.53 | 3.79 | 101.4 | 3.53 |
| 35 | y = 300,359x – 36,234 | 0.9908 | 4.100–4.001 × 104 | 0.57 | 2.0 | 3.32 | 3.63 | 4.04 | 3.54 | 104.4 | 4.14 |
| 36 | y = 1.3913x + 3.9365 | 0.9976 | 2.810 × 104–2.160 × 107 | 3.5 × 103 | 14 × 103 | 1.73 | 2.12 | 2.42 | 2.67 | 102.4 | 3.04 |
| 37 | y = 1.3796x + 4.2746 | 0.9953 | 5.350 × 104–4.108 × 107 | 6.7 × 103 | 13 × 103 | 1.89 | 2.17 | 2.75 | 2.32 | 98.7 | 3.02 |
| 38 | y = 1.4068x + 3.8994 | 0.9988 | 2.680 × 104–2.060 × 107 | 3.4 × 103 | 13 × 103 | 1.90 | 2.42 | 2.48 | 3.03 | 101.4 | 2.97 |
| 39 | y = 1.3851x + 5.4207 | 0.9964 | 2.420 × 104–4.650 × 107 | 3.0 × 103 | 12 × 103 | 2.43 | 2.55 | 3.14 | 2.96 | 98.4 | 3.08 |
| 40 | y = 1.4024x + 1.8305 | 0.9986 | 9.980 × 104–5.474 × 107 | 12 × 103 | 25 × 103 | 2.11 | 2.53 | 2.16 | 2.75 | 101.9 | 3.25 |
| 41 | y = 1.4189x + 5.0747 | 0.9977 | 5.190 × 104–4.980 × 107 | 6.5 × 103 | 13 × 103 | 1.83 | 2.43 | 2.43 | 2.31 | 99.9 | 2.94 |
| 42 | y = 1.4088x + 4.1731 | 0.9988 | 2.800 × 104–2.148 × 107 | 3.5 × 103 | 14 × 103 | 1.69 | 1.93 | 2.31 | 2.01 | 100.4 | 2.88 |