Figure 3.

Modulation of CD45RA expression on T cells by MSC-EVs. (a,b) Effect of four different mesenchymal stem/stromal cells–extracellular vesicles (MSC-EV) preparations (EV1–EV4) on the frequency of CCR7 and CD45RA expressing T cells and (c–f) capacity to modulate CD4⁺ and CD8⁺ effector (T_E) and effector memory (T_EM) T cell subsets upon 4 h of PMA/Ionomycin stimulation. Frequencies of cell populations (%) are presented as median with minimum and maximum. Black dotted line indicates the median frequency of a certain population obtained without PMA/Ionomycin stimulation; red dotted line indicates the median frequency of a certain population obtained after PMA/Ionomycin stimulation in the absence of EV. Statistical analysis was performed by Wilcoxon test.