Figure 2.

dsRNA-induced EGFP and NPTII transgene suppression in four-week-old Arabidopsis thaliana. The expression levels (log2 fold change) of the NPTII (a) and EGFP (b) in response to external application of sterile filtered water (Control), NPTII-dsRNA, and EGFP-dsRNAs relative to NPTII and EGFP levels in the plants before treatments. The dsRNA were diluted in water to 0.35 µg/µL (100 µL per plant). The RNA was isolated before, 1, 7, and 14 days post-treatment from an individual plant of each line per treatment (four independent experiments). qRT-PCR data are presented as mean ± SE. *, **—significantly different from the untreated plants at P ≤ 0.05 and 0.01, respectively, according to the Student’s t-test.