Figure 3.

The analysis of NPTII (a) and EGFP (b) transgene transcript levels (log2 fold change) using primers designed to align inside the NPTII and EGFP transgene fragments, which have been used for synthesis of the corresponding dsRNAs, in the dsRNA-treated four-week-old Arabidopsis thaliana relative to that in the plants before treatments. One plant of the L1, L2, and L3 lines for each treatment was treated with NPTII-dsRNA, EGFP-dsRNAs or filtered sterile water (Control). The dsRNA were diluted in water to 0.35 µg/µL (100 µL per plant). In an experiment, the RNA was isolated before, 1, 7, and 14 days post-treatment from each plant (three independent experiments). qRT-PCR data are presented as mean ± SE. *, **—significantly different from the plants before treatment at P ≤ 0.05 and 0.01, respectively, according to the Student’s t-test.