Table 1.
Phytochemical composition (%), identification and major groups of chemical components of the essential oil of Micromeria croatica (Pers.) Schott from the localities Bojinac (Boj), Bačić kuk (Bk) and Stupačinovo (St) *.
| Sample (Yield in %) c | |||||
|---|---|---|---|---|---|
| Component | RI a | RI b | Micromeria croatica | ||
| Boj ± SD (1.3) | Bk ± SD (0.7) | St ± SD (0.6) | |||
| Monoterpene hydrocarbons | 21.8 | 24.5 | 14.0 | ||
| α-Thujene | 924 | 1035 | - | 0.9 ± 0.03 | - |
| α-Pinene ** | 938 | 1032 | 0.5 ± 0,15 | 1.1 ± 0.01 | 1.0 ± 0.01 |
| Camphene ** | 962 | 1059 | 0.9 ± 0.01 | 0.4 ± 0.01 | 0.6 ± 0.01 |
| 1-Octen-3-ol | 974 | 1452 | 1.2 ± 0.05 | 1.1 ± 0.01 | 0.3 ± 0.01 |
| Myrcene | 992 | 1174 | 2.4 ± 0.01 | 1.4 ± 0.01 | 0.8 ± 0.01 |
| δ-3-Carene | 1008 | 1153 | 2.5 ± 0.01 | 1.8 ± 0.01 | 0.6 ± 0.1 |
| α-Terpinene | 1016 | 1179 | 2.7 ± 0.01 | 4.2 ± 0.05 | 5.1 ± 0.01 |
| p-Cymene | 1021 | 1268 | 1.5 ± 0.05 | 3.3 ± 0.01 | 1.3 ± 0.06 |
| Limonene | 1032 | 1204 | 3.9 ± 0.06 | 5.9 ± 0.01 | 0.8 ± 0.01 |
| (Z)-β-Ocimene ** | 1032 | 1218 | 0.4 ± 0.01 | 1.6 ± 0.01 | 0.9 ± 0.01 |
| γ-Terpinene | 1057 | 1255 | 3.5 ± 0.03 | 2.6 ± 0.01 | 1.6 ± 0.01 |
| allo-Ocimene | 1128 | 1351 | 2.3 ± 0.01 | 0.9 ± 0.01 | 1.0 ± 0.03 |
| Oxygenated monoterpenes | 25.8 | 41.3 | 27.6 | ||
| Linalool oxide ** | 991 | - | 0.9 ± 0.01 | 0.8 ± 0.01 | 0.5 ± 0.01 |
| Sabinene hydrate | 1065 | 1474 | 1.1 ± 0.04 | 0.7 ± 0.01 | 0.4 ± 0.01 |
| Terpinolene | 1085 | 1286 | 1.6 ± 0.03 | 2.4 ± 0.1 | 0.4 ± 0.07 |
| Linalool | 1097 | 1548 | 3.5 ± 0.03 | 6.4 ± 0.01 | 1.1 ± 0.01 |
| α-Campholenal | 1122 | - | 1.8 ± 0.01 | 1.2 ± 0.01 | 0.7 ± 0.02 |
| cis-Verbenol | 1137 | - | 0.2 ± 0.01 | 0.7 ± 0.03 | 0.5 ± 0.01 |
| Camphor | 1143 | 1499 | 1.9 ± 0.01 | 3.1 ± 0.01 | 6.8 ± 0.01 |
| Borneol | 1165 | 1719 | 2.2 ± 0.03 | - | 0.2 ± 0.01 |
| Terpinen-4-ol | 1174 | 1611 | 1 ± 0.01 | 2.5 ± 0.01 | 0.7 ± 0.01 |
| α-Terpineol | 1186 | 1646 | 1.1 ± 0.01 | 2.1 ± 0.01 | 0.5 ± 0.1 |
| Myrtenol | 1194 | 1804 | 1.4 ± 0.03 | 2.5 ± 0.01 | 3.6 ± 0.03 |
| trans-Carveol | 1216 | - | 1 ± 0.01 | 1.3 ± 0.01 | - |
| Nerol | 1227 | 1808 | 2 ± 0.04 | 0.6 ± 0.01 | 1.2 ± 0.01 |
| Thymol methyl ether | 1230 | 1604 | 0.5 ± 0.01 | 3.3 ± 0.01 | 0.8 ± 0.01 |
| Carvacol methyl ether | 1241 | 1614 | 0.2 ± 0.01 | 1.1 ± 0.01 | 2.1 ± 0.01 |
| Geraniol | 1249 | 1857 | 0.8 ± 0.01 | 5.1 ± 0.01 | 0.6 ± 0.01 |
| Isobornyl acetate | 1283 | - | 0.4 ± 0.01 | 1.7 ± 0.01 | 0.2 ± 0.04 |
| Thymol | 1290 | - | 1.2 ± 0.01 | 0.2 ± 0.01 | 1.1 ± 0.01 |
| Carvacrol | 1298 | 2239 | 2.2 ± 0.01 | 0.7 ± 0.01 | 0.4 ± 0.03 |
| Thymol acetate | 1349 | - | - | - | 5.4 ± 0.01 |
| Neryl acetate | 1358 | 1692 | 0.8 ± 0.1 | 4.9 ± 0.01 | 0.4 ± 0.03 |
| Sesquiterpene hydrocarbons | 31.4 | 5.0 | 16.3 | ||
| α-Cubebene | 1345 | - | 3.5 ± 0.07 | 1.4 ± 0.01 | 2.3 ± 0.01 |
| β-Caryophyllene ** | 1417 | 1585 | 25.2 ± 0.03 | 1.8 ± 0.01 | 10.2 ± 0.01 |
| β-Copaene | 1430 | 1457 | 1 ± 0.01 | 0.7 ± 0.01 | 1.4 ± 0.01 |
| Aromadendrene | 1439 | - | 0.7 ± 0.02 | 0.3 ± 0.01 | 1.9 ± 0.1 |
| δ-Cadinene | 1517 | 1745 | 1 ± 0.01 | 0.8 ± 0.01 | 0.5 ± 0.01 |
| Oxygenated sesquiterpenes | 15.0 | 21.6 | 32.9 | ||
| Sphatulenol | 1578 | 2144 | 1.6 ± 0.04 | 0.5 ± 0.01 | 2.2 ± 0.01 |
| Caryophyllene oxide | 1582 | 1927 | 10.1 ± 0.08 | 21.1 ± 0.01 | 20.2 ± 0.01 |
| Viridiflorol | 1592 | - | 3.3 ± 0.01 | - | 1.1 ± 0.01 |
| β-Bisabolol | 1674 | 2166 | - | - | 3.0 ± 0.01 |
| Caryophyllene acetate ** | 1701 | - | - | - | 4.9 ± 0.1 |
| Farnesol | 1742 | - | - | - | 1.5 ± 0.01 |
| Total identified (%) | 94.0 | 92.4 | 90.8 | ||
Retention indices (RI) were determined relative to a series of n-alkanes (C8–C40) on capillary columns VF5-ms (RI a) and CP Wax 52 (RI b); c average value from two columns, where possible; * identification method: RI, comparison of RIs with those listed in a homemade library, reported in the literature [30], and/or authentic samples; comparison of mass spectra with those in mass spectral libraries NIST02 and Wiley 9; ** co-injection with reference compounds; SD, standard deviation.