Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Feb 20;23:60–74. doi: 10.1016/j.molmet.2019.02.004

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

figs3 — Figure S3. (A) Cell size quantification of adipocytes in rWAT from of 6 weeks old myf5-Cre;R26R-mTmG (grey circles), Akt1^myf5cKO;R26R-mTmG (blue squares) and Akt2^myf5cKO;R26R-mTmG mice (Akt2 deficient as green triangles and Akt2 wildtype as red circles). The number of adipocytes analyzed are: n = 187 for myf5-Cre;R26R-mTmG, n = 152 for Akt1^myf5cKO;R26R-mTmG and n = 219 GFP⁺ and 82 Tomato⁺ for Akt2^myf5cKO;R26R-mTmG. One-way ANOVA was used for statistical analysis. (B) Percentage (average ± SD) of mTFP+ (Red) and mGFP+ (Green) APCs isolated from the indicated depots from 6 weeks old myf5-Cre; R26R-mTmG, Akt1^myf5cKO;R26R-mTmG and Akt2^myf5cKO;R26R-mTmG mice (n = 3 for myf5-Cre; R26R-mTmG, 2 for Akt1^myf5cKO;R26R-mTmG and 4 for Akt2^myf5cKO;R26R-mTmG; no statistical analysis was used). A total of 50000 singles were used for this analysis. (C) Western blot of Akt2^myf5cKO and control (Akt2 floxed) rWAT after overnight fasting with or without refeeding. (D) Western blot of Akt1^myf5cKO, Akt2^myf5cKO and control (Akt1 floxed and Akt2 floxed, respectively) quadriceps after overnight fasting with or without refeeding.