Table 1.
Reagents used in three versions of the urine treatment and distribution of recovery results.
| Item | Method A | Method B | Method C |
| Reagents | |||
| Extraction Solvent | 400 μL of acetonitrile | 1% HOAc in acetonitrile (400 μL) | 400 μL of acetonitrile |
| Extraction Reagent | MgSO4 (40 mg) NaCl (10 mg) |
MgSO4 (40 mg) NaOAc (10 mg) |
MgSO4 (40 mg) NaCl (10 mg) Na3Citrate (10 mg) Na2HCitr (5 mg) |
| Recovery (%) | No. of Analytes (%) | No. of Analytes (%) | No. of Analytes (%) |
| 40–70 (RSD ≤ 20%) | 2 (0.8%) | 3 (1.2%) | 0 |
| 70–120 (RSD ≤ 20%) | 258 (99.2%) | 256 (98.5%) | 258 (99.2%) |
| 70–120 (RSD > 20%) | 0 | 0 | 1 (0.4%) |
| >120 (RSD ≤ 20%) | 0 | 1 (0.4%) | 0 |
| n.d.1 | 0 | 0 | 1 (0.4%) |
| Sum | 260 (100%) | 260 (100%) | 260 (100%) |
| Average Recovery | 92.3% | 87.3% | 84.9% |
1 Not determined.