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. 2019 Apr 1;20(7):1615. doi: 10.3390/ijms20071615

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Functional classification, subcellular localization, and hierarchical clustering of the 60 DAPs in pollen grain of the control and CHA-SQ-1-treated wheat plants. The DAPs were divided into nine functional groups (A) and classified by predicted subcellular localization (B) using Euk-mPLoc 2.0 database (http://www.csbio.sjtu.edu.cn/bioinf/euk-multi-2/), BaCelLo database (http://gpcr.biocomp.unibo.it/bacello/pred.htm) and ESLpred database (http://www.imtech.res.in/raghava/eslpred2/). The black bars indicate high-abundant proteins and the white bars low-abundant proteins. (C) The hierarchical cluster analysis was conducted using the Genesis 1.7.6 procedure (Graz University of Technology, Austria, http://genome.tugraz.at/genesisclient/genesisclient_download.shtml) and the log₂-transformed values of -fold change ratios listed in Table S1.