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. 2019 Apr 2;20(7):1644. doi: 10.3390/ijms20071644

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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The source of bradykinin-induced reactive oxygen species is NADPH oxidases. (A,B) Time course of changes in C-DCDHF-DA intensity, and summarized data of C-DCDHF-DA intensity at 180 s and 360 s in the presence (open diamond; n = 336) or absence (closed circle; n = 300) of VAS2870. (C,D) The same experiment as (A) conducted in the presence (open diamond; n = 308) or absence (closed circle; n = 201) of apocinin. Data are expressed as the mean ± SEM of 3–5 independent experiments in separate cell culture wells. * p < 0.05 versus BK. Two-factor factorial ANOVA was used for analysis of difference.