Figure 5.

NADPH oxidase-derived reactive oxygen species production by bradykinin is mediated via a Ca²⁺/Calmodulin-dependent pathway. (A,B) Time course of changes in C-DCDHF-DA intensity, and summarized data of C-DCDHF-DA intensity at 180 s and 360 s after BK application in the presence (open diamond; n = 93) or absence (open circle; n = 136) of W-7. (C,D) The same experiments as (A) conducted in the presence (open diamond; n = 63) or absence (open circle; n = 136) of KN-93. Data are expressed as the mean ± SEM of two independent experiments in separate cell culture wells. * p < 0.01 versus BK. Two-factor factorial ANOVA was used for analysis of difference.