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Biochemical characterization. (a) Activity in the presence of ACN; (b) pH stability and (c) thermostability after a 1 h incubation (the value for 100 % of activity was taken as the initial activity of each enzyme without incubation at room temperature, 25 °C). Activity was measured in sodium phosphate/citrate buffer (pH 4.4, 100 mM) containing ABTS (0.3 mM) and H2O2 (2 mM). Black bars, PaDa-I-Cys immobilized on C-II; grey bars, free PaDa-I-Cys; white bars, PaDa-I.
