Table 5.
Details of cell culture treatments.
Compound | Catalog Number | Function | Concentration | Exposure Time | Experiment |
---|---|---|---|---|---|
TNF-α | 315-01A PeproTech | Inflammatory cytokine | 5, 10 ng/mL | 18 h | qPCR Ca imaging |
IL-1β | 200-01B PeproTech | Inflammatory cytokine | 5, 10 ng/mL | 18 h | qPCR Ca imaging |
5 ng/mL | 1, 8, 15 days | qPCR (from 3D) | |||
5 ng/mL | 18 h | Immuno | |||
AITC | 377430 Sigma | TRPA1 agonist | 3 μM, 10 μM | 18 h | qPCR, ELISA |
100 μM | during the measurement | Ca imaging |