Figure 5.

ELISA was used to quantify the level of proinflammatory cytokines; IL-1β, IL-6 and TNF-α in the mid brain tissues of rats from different experimental groups. The levels of IL-1β (A), IL-6 (B) and TNF-α (C) were significantly (*** p < 0.001) enhanced in ROT challenged rats when compared to CONT group rats. Thymol treatment to ROT challenged rats showed a significant (## p < 0.01; # p < 0.05) decrease in the levels of ROT-induced rise of proinflammatory cytokines. Additionally, the cytokines levels did no show significant difference in the rats of CONT and thymol alone groups. The values are presented as mean ± SEM (n = 6–8).