Figure 3.

Oxygen tension-dependent expression of prototypic TGF-β ligand isoforms. We used RT-qPCR to screen oxygen-dependent changes in mRNA abundance of all three human TGF-β isoforms, TGFB1-3 (A). As TGFB2 was most prominently upregulated by extracellular oxygenation levels of 2.5% pO₂, and below, we validated its expression on protein level (B), with its abundance in 20% pO₂ set to 1. Oxygenation levels (% pO₂) appear as grey scales as shown within the figure. RT-qPCR (n = 6), ELISA (n = 3); * p < 0.05, ** p < 0.01, *** p < 0.001.