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Oxygen tension-dependent abundances of TGF-β receptors. Normalized relative mRNA expression of TGF-β type I (ALK1, ALK5) and type II (TGFBR2) receptors (A). Representative images of TGF-β type I and type III (betaglycan, BGCN; endoglin, EGN) receptor protein abundances. Chondrocytes were cultured under 1% and/or 2.5% and 20% pO2, respectively; loading control, β-actin (B). MW in kDa on right. RT-qPCR (n = 5), immunoblots (n = 3); ** p < 0.01.
