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Purification and molecular weight determination of BGAP: (a) cation exchange chromatography of crude protein extract on SP-Sepharose column: (−): absorbance at 280 nm, (…): concentration of NaCl; (b) cation exchange chromatography of active fraction SP3 from SP-Sepharose column on Mono S column; (c) gel filtration chromatography of active fraction MS3 from Mono S column on Superdex peptide column; (d) Tricine-SDS-PAGE of active fraction SU3 from Superdex peptide column.
