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. 2019 Apr 5;20(7):1705. doi: 10.3390/ijms20071705

Figure 1.

Figure 1

Viable and non-viable microspores and pollen grains of common buckwheat cv. ‘Panda’ and strain PA15 under control (20 °C) and thermal stress (30 °C) conditions. Vacuolated microspores of ‘Panda’ at 20 °C with thick sporodermis (a, arrow); viable pollen grains of PA15 at 20 °C stained with Alexander dye (b) and at 30 °C stained with Ehrlich’s hematoxylin combined with Alcian blue (c); and viable and non-viable (arrows) pollen grains of ‘Panda’ at 30 °C stained with fluorescein diacetate (d) and at 20 °C stained with Ehrlich’s hematoxylin combined with Alcian blue (e). At least 7900 pollen grains per treatment were analyzed in two replicates.