Skip to main content
. 2019 Mar 13;23:51–59. doi: 10.1016/j.molmet.2019.03.002

Figure 4.

Figure 4

RYGB affects the expression of key genes implicated in lipid transport and absorption, in lipid droplet storage and FAO. (A) Enterocyte gene expression levels of genes related to lipid absorption, resynthesis, secretion, or storage in the fasted state. [n = 6/8; Student t-tests: Fatp4: Fatty acid transport protein 4, P < 0.05; Fabp2: fatty acid binding protein 2, P < 0.05; Cd36: fatty acid translocase, P < 0.05; Acsl5: acyl-CoA synthetase long-chain family member 5, P < 0.05; Cpt1: Carnitine palmitoyltransferase 1, P = 0.4026; Mgat1: Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase, P < 0.05; Dgat1: Diglyceride acyltransferase 1, P < 0.05; Mtp: Microsomal triglyceride transfer protein, P = 0.1578; Apob: Apolipoprotein B, P < 0.05; Apoa4: Apolipoprotein A-IV, P < 0.05]. Data are presented as mean values ± SEM. (B–C) FAU and FAO in isolated enterocytes in a fasted and fed (30 min after a TM) state [n (biological) = 3; n (technical) = 6/8; Student t-tests: FAU and FAO: P fasted < 0.0005; P fed < 0.005]. (D) ORO stained intestinal cross-sections of a representative Sham animal (A) and a representative RYGB animal (B) [n = 3/4, ×50 magnification] after an overnight fast. Higher villus area for RYGB animals, P < 0.0001. (E) Red pixel % [n = 3/4, P = 0.5060].