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. Author manuscript; available in PMC: 2019 Aug 1.
Published in final edited form as: Fungal Genet Biol. 2018 May 12;117:21–29. doi: 10.1016/j.fgb.2018.05.003

Fig. 2.

Fig. 2

Protein purification and in vitro cleavage activity assay. (A) SDS-PAGE gel depicting protein expression and purification conditions. Lane 1 represents total protein before inducing; Lane 2 total protein after addition of 0.3 mM IPTG for 5 h; Lane 3 represents about 5 μg purified NLSH2BCas9 protein. (B) Top: diagram depicting the template length, the sgRNA cleavage site, and the lengths of expected fragments after cleavage with Cas9; Bottom: assessment of the cleavage efficiency by gel electrophoresis after incubation at 37 °C for one hour.