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. Author manuscript; available in PMC: 2019 Aug 1.
Published in final edited form as: Fungal Genet Biol. 2018 May 12;117:21–29. doi: 10.1016/j.fgb.2018.05.003

Fig. 5.

Fig. 5

FoBIK1 gene encoding a polyketide synthase in a secondary metabolite biosynthetic cluster is the core enzyme responsible for the biosynthesis of a red pigment. (A) The BIK1 gene structure, the location of the sgRNA in exon 1, and the construction of the associated HDR template. (B) The cleavage efficiency of the sgRNA for bik1 in vitro after incubation at 37 °C for one hour. (C) The phenotypes of three replicative wild type and six randomly chosen bik1 mutants cultured for seven days. (D) HPLC-DAD analysis of red pigment production between wild type and bik1 mutants.