. Author manuscript; available in PMC: 2019 Aug 1.

Published in final edited form as: Fungal Genet Biol. 2018 May 12;117:21–29. doi: 10.1016/j.fgb.2018.05.003

Table 1.

Mutation frequencies of homologous directed repair using Cas9 RNPs

Gene	Donor DNA^a	Cas9/sgRNA^a	Pick No.	Positive No.	Frequency (%)
URA5	6	0/0	32	0	0
	6	10/2	14	3	21.4

bik1	6	0/0	27	0	0
	6	5/1	20	4	20
	6	10/2	13	7	53.8
	6	15/3	25	12	48
	6	20/4	21	2	9.5

number of μg used of each per reaction.