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. Author manuscript; available in PMC: 2020 Feb 7.
Published in final edited form as: Mol Cell. 2018 Dec 27;73(3):601–610.e5. doi: 10.1016/j.molcel.2018.11.016

Figure 1. AcrIIA2 Selectively Forms a Stable Complex with the sgRNA-Loaded S. pyogenes Cas9.

Figure 1.

(A) Flow chart for reconstitution and isolation of the AcrIIA2-bound SpyCas9-sgRNA ternary complex using ultrafiltration combined with size-exclusion chromatography (UF-SEC).

(B) Size-exclusion chromatogram ofSpyCas9-AcrIIA2 in the presence or absence of sgRNA. The inset representsthe calibration curve obtained using standard molecular weight markers.

(C) Coomassie-stained polyacrylamide gel showing the co-purification of AcrIIA2 with sgRNA-bound SpyCas9.