Figure 4.

Rosiglitazone induces browning characteristics during adipocytes formation. (A) Oil-O red staining of adipocytes with (+) or without (−) rosiglitazone. (B) Gene expression profiles of EBF2 transcription factor and UCP-1 brown adipocyte marker in control and 2 µM rosiglitazone treatment in the induction (+2 µM-M) and rosiglitazone treatment within both induction and maintenance (+2 µM+M). (C) The representative Western blot of brown adipocyte marker, UCP-1, in the differentiated adipocytes without rosiglitazone and with rosiglitazone in the induction only and rosiglitazone in induction and maintenance: UCP-1 (~32 kDa), normalized to that of β-actin (~45 kDa). (D) Quantity of the UCP-1 protein level in each condition. The data are presented as mean ± S.E.M. * p < 0.05, ** p < 0.01 versus control.