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. 2019 Apr 1;20(7):1618. doi: 10.3390/ijms20071618

Figure 5.

Figure 5

MAP kinase and PI3-kinase signaling pathways activated by rosiglitazone during the enhancing of brown lineage. Total and phosphorylated proteins of derived adipocytes from different experimental groups without (−) (control) or with (+) 2 µM rosiglitazone 48 hours (+2 µM+M) or 96 h (+2 µM-M): (A) MAPK (~42/44 kDa) and phospho-MAPK (~42/44 kDa); (B) total AKT (~60 kDa), phospho-AKT-serine (~60 kDa), and phospho-AKT-threonine (~60 kDa) normalized to that of β-actin (~45 kDa). Statistical data are presented as mean ± S.E.M. *p < 0.05, **p < 0.01, ***p < 0.001 versus control.