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. 2019 Mar 31;20(7):1608. doi: 10.3390/ijms20071608

Figure 4.

Figure 4

Knockdown of GHR reduced the GH binding activity and downregulated the expression of key regulators of mitochondrial biogenesis and mtDNA-encoded genes in DF-1 cells. (A) Transfection efficiency was measured by the fluorescence intensity of FAM siRNA and qRT-PCR at 48 h after transfection with si-GHR and si-NC fragments in DF-1 cells. Bar, 100 μm. The relative mRNA expression of genes involved in the GH–GHR–IGF1 signaling pathway (B) and PGC1α–NRF1–TFAM signaling pathway (C) were measured by qRT-PCR at 48 h after transfection with si-GHR and si-NC fragments in DF-1 cells. (D) mtDNA transcription was measured by qRT-PCR at 48 h after transfection with si-GHR and si-NC fragments in DF-1 cells. Data are expressed as means ± SEM, * p < 0.05; ** p < 0.01; *** p < 0.001.