Table 3.
Number of predicted potential bioactive peptides to be released from identified proteins of C. sorokiniana using BIOPEP’s “enzyme action” tool.
| Protease | Chloroplast Rubisco Activase | Phosphoglycerate Kinase | Heat Shock Protein 70 | ATP Synthase Subunit Beta | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AC | AO | S | DP | AC | AO | S | DP | AC | AO | S | DP | AC | AO | S | DP | |
| Pepsin (EC 3.4.23.1) | 31 | 2 | 2 | 48 | 29 | - | 5 | 51 | 42 | 11 | 1 | 78 | 44 | 6 | 9 | 66 |
| Thermolysin (EC 3.4.24.27) | 12 | - | - | 14 | 20 | 4 | - | 23 | 21 | - | - | 31 | 20 | 1 | - | 25 |
| Bromelain (EC 3.4.22.32) | 26 | 3 | 1 | 36 | 25 | 8 | 3 | 41 | 36 | 3 | 2 | 50 | 20 | 2 | 4 | 37 |
| Chymotrypsin A (EC 3.4.21.1) | 5 | 1 | 1 | 10 | 9 | 1 | 2 | 11 | 8 | 2 | 2 | 15 | 7 | 4 | 2 | 10 |
| Chymotrypsin C (EC 3.4.21.2) | 17 | 3 | 1 | 16 | 14 | - | 2 | 19 | 20 | 6 | 2 | 35 | 17 | 3 | 4 | 25 |
| Pancreatic elastase (EC 3.4.21.36) | 16 | 2 | - | 31 | 27 | 5 | - | 49 | 21 | 2 | - | 34 | 19 | 2 | - | 41 |
| Papain (EC 3.4.22.2) | 31 | 3 | 1 | 35 | 27 | 6 | 2 | 31 | 40 | 4 | 4 | 50 | 27 | 1 | 4 | 43 |
| Proteinase K (EC 3.4.21.67) | 12 | 1 | - | 20 | 17 | 1 | 1 | 19 | 23 | 5 | - | 41 | 15 | 1 | - | 28 |
| Trypsin (EC 3.4.21.4) | 5 | - | - | 3 | 3 | 1 | - | 3 | 5 | - | - | 2 | 3 | - | - | 3 |
| Ficin (EC 3.4.22.3) | 27 | 7 | 2 | 37 | 22 | 8 | 3 | 33 | 26 | 4 | 3 | 35 | 22 | 7 | 1 | 28 |
| Cathepsin (EC 3.4.21.20) | 4 | 1 | 1 | 6 | 7 | 1 | 1 | 6 | 6 | 2 | 1 | 7 | 5 | 3 | 1 | 6 |
| Subtisilin (EC 3.4.21.62) | 10 | 4 | 2 | 14 | 10 | 3 | 4 | 18 | 9 | 5 | 7 | 24 | 12 | 2 | 6 | 23 |
| Pepsin + Trypsin | 28 | 4 | 2 | 55 | 33 | 1 | 5 | 56 | 36 | 2 | 10 | 66 | 40 | 6 | 9 | 62 |
| Pepsin + Trysin + Chymotrypsin A | 31 | 4 | 1 | 40 | 33 | 1 | 5 | 54 | 34 | 1 | 10 | 63 | 38 | 5 | 9 | 61 |
| Pepsin + Trysin + Chymotrypsin C | 28 | 4 | 1 | 33 | 35 | 1 | 4 | 54 | 41 | 2 | 10 | 70 | 38 | 5 | 9 | 51 |
Abbreviation: ACE Inhibitory (AC), Antioxidant (AO), Stimulating (S), DPP IV inhibitory (DP).