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Ponatinib induced cell apoptosis in SK-Hep-1 and SNU-423 cells in a concentration-dependent manner. (A) SK-Hep-1 and SNU-423 cells were stained with Hoechst 33342 observed on fluorescence microscopy (×200). (B) Flow cytometry analysis of apoptosis (data from three independent experiments). (C) Western blot analysis of cleaved caspase-3, cleaved caspase-7, Bax, Bcl-2, and β-Tubulin (control) in treated cells.
