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. 2019 Apr 3;20(7):1661. doi: 10.3390/ijms20071661

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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IMM-H004 protects against Permanent focal cerebral ischemia-induced cardiopulmonary complications in aged rats. (A) Representative photographs of HE-stained heart and lung subfield from aged rats (400× magnification, scale bar: 50 μm). (B) Effect of IMM-H004 on LDH level in heart of aged rats using an LDH assay kit. (C) Expression of CKLF1 in heart and lung subfield from aged rats by immunohistochemical staining (50× and 400× magnification, scale bar: 500 and 50 μm). All the data are shown as the mean ± SD; part of data are shown as the mean ± SD after normalization to the sham (n = 6/group). ## p < 0.01 vs. sham; ### p < 0.001 vs. sham; ** p < 0.01 vs. model; *** p < 0.001 vs. model.