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. 2019 Apr 3;20(7):1661. doi: 10.3390/ijms20071661

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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IMM-H004 decreases inflammation in heart and lung of aged rats. (A) Effect of IMM-H004 on aged rats heart and lung mRNA levels of CKLF1, CCR4, IL-1β, and TNF-α as determined by quantitative RT-PCR. (B) Effect of IMM-H004 on the CKLF1, CCR4, IL-1β, and TNF-α protein expression levels in aged rats heart and lung as assayed by Western blotting using a Gel-Pro analyzer (Media Cybernetics, Rockville, MD, USA). All the data are shown as the mean ± SD; part of data are shown as the mean ± SD after normalization to the sham (n = 6/group). ### p < 0.001 vs. sham; * p < 0.05 vs. model; *** p < 0.001 vs. model.