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. 2019 Mar 31;16(7):1157. doi: 10.3390/ijerph16071157

Figure 1.

Figure 1

GFP fluorescence and ACSL-5 overexpression with transfection in primary human skeletal muscle cells (10X magnification). (A) Primary human skeletal myoblasts were transfected using positive control vector GFP without ACSL-5 plasmid DNA to verify transfection. Results indicated an approximately 50% transfection efficiency at 24 h after transfection. (B) At day 7 of the differentiation period, GFP fluorescence was strongly expressed in the primary human skeletal muscle cells. (C) Transfection efficiency of ACSL-5 normalized to GAPDH (open bar, control cells; closed bar, ACSL-5 transfected cells). The protein levels of ACSL-5 were approximately 2-fold higher in ACSL-5 overexpressed cells than in control. Data are presented as mean ± SEM (n = 6). * p < 0.05 versus control. Blots were probed with antibodies recognizing ACSL-5 or GAPDH. (D,E) Effect of transfection on cell morphology (10× magnification). ACSL-5 overexpression did not affect differentiation in human skeletal muscle cells based on the morphology of control (D) and ACSL-5 overexpressed cells (E).