Figure 2.

Effect of ACSL-5 overexpression on fatty acid oxidation. Fatty acid oxidation was measured using ¹⁴C-labeled radioactivity of CO₂ (A) and ASM (B) via liquid scintillation counting (open bar, control cells; closed bar, ACSL-5 transfected cells). Palmitate complete oxidation (CO₂ producton) was approximately 2-fold higher in the ACSL-5 transfected group compared with the GFP-only transfected control (A). Similarly, palmitate incomplete oxidation (ASM production) was significantly increased by ACSL-5 overexpression (B). Data are expressed as presented as mean ± SEM (n = 6). * p < 0.05 versus control.