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Effect of ACSL-5 overexpression on insulin signaling. Protein levels of phospho-/total IRS-1 (A), phospho-/total Akt (B), and phospho-/total AS160 (C) were measured using Western immunoblot analysis (open bar, control cells; closed bar, ACSL-5 transfected cells). Western blots and mean (±SEM) data (n = 6) in the absence or presence of insulin treatment were presented. a p < 0.05 versus non-insulin-treated control; b p < 0.05 versus insulin-treated control (CON).
