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. 2019 Apr 3;24(7):1316. doi: 10.3390/molecules24071316

Figure 2.

Figure 2

pH-zone-refining counter-current chromatography (pH-ZRCCC) chromatogram of the crude sample of E. yunnanensis. (a) hexane–ethyl acetate–methanol–water (3:7:1:9, v/v), 0.7g of sample, 10 mM triethylamine (TEA) in upper phase, and 10 mM HCl in lower phase; (b) hexane–ethyl acetate–methanol–water (5:5:2:8, v/v), 0.7g of sample, 10 mM TEA in upper phase, and 10 mM HCl in lower phase; (c) hexane–ethyl acetate–methanol–water (5:5:2:8, v/v), 0.8g of sample, 20 mM TEA in upper phase, and 5 mM HCl in lower phase; (d) hexane–ethyl acetate–methanol–water (5:5:2:8, v/v), 2.1 g of sample, 20 mM TEA in upper phase, and 5 mM HCl in lower phase. Experimental conditions: revolution speed: 850 r/min; flow rate: 2.0 mL/min; UV detection wavelength: 254 nm.