Fig. 2.

Human T cell biomanufacturing development. a Effect of basal media on T cell growth. b Cell proliferation with the seeding culture collected from Days 0, 2 and 5. c Proliferation capability of cells post 1st stimulation, 2nd stimulation and freeze/thaw. d Re-stimulation with anti-CD3/CD28 mAbs coated on solid beads or mAbs tetramer. OpTmizer basal medium was used and 30 IU/mL IL-2 was fed on Days 0, 2, and 4. *p < 0.05, *p < 0.01. P-values were analyzed by one-way ANOVA. The error bars are presented as standard error of the mean (SEM)