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. 2019 Apr 23;13:34. doi: 10.1186/s13036-019-0167-2

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Quality of the T cells in our novel biomanufacturing. a Diagram of T cell proliferation and differentiation pathway. b Both CD4⁺ and CD8⁺ T cells showed high 99% purity after the expansion. c Proliferation signal. PD-1 expression level was related to cell proliferation status. Note: The CCR7, CD45RA and CD45RO surface proteins were used to identify cell subsets [57]