Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Apr 23;19:375. doi: 10.1186/s12885-019-5582-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

Fig. 1 — Identification of HPV integration site in 13q22.1 in both cervical and ovarian tumors, case 1. a The junction between HPV18 (grey box) and human genome is indicated by a vertical grey line. The coordinate of the human genome breakpoint (73884335) in cervical tumor was determined according to the forward strand (+) of hg19 reference. Horizontal arrows indicate the localization of the primers, which were designed to specifically amplify the human/viral junction sequence. The closest genes (KLF5, KLF12) to the HPV integration site are shown as black boxes. b Analysis of the PCR products on agarose gel. Lane 1 represents molecular weight, lanes 2 and 3 indicate cervical and ovarian tumor DNA, respectively. Lane 4 is DNA from another patient (negative control). c Sanger sequencing chromatogram obtained from cervical tumor DNA (upper part) and ovarian tumor DNA (lower part). Vertical grey line indicates the junction between HPV18 (left part) and the human genome (right part)