Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Apr 23;19:375. doi: 10.1186/s12885-019-5582-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

Fig. 2 — Identification of the HPV integration site in 2q22.3 in both cervical and ovarian tumors, case 2. a The junction between HPV18 (grey box) and the human genome is indicated by a vertical grey line. The coordinate of the human genome breakpoint (146477121) in the cervical tumor was determined according to the forward strand (+) of the hg19 reference. Horizontal arrows indicate the sites of the primers designed to specifically amplify the human/viral junction sequence. The genes closest to the HPV integration site are identified at 643 kb (TEX41) and 2 Mb (ACVR2) and shown as black boxes. b Analysis of the PCR products on agarose gel. Lane 1 represents molecular weight, lanes 2 and 3 indicate cervical and ovarian tumor DNA, respectively. Lane 4 is DNA from another patient (negative control). c Sanger sequencing chromatogram obtained from cervical tumor DNA (upper part) and ovarian tumor DNA (lower part). The vertical grey line indicates the junction between HPV18 (left part) and the human genome (right part)