Fig. 5.

piRNA transcripts increase with age in egg chambers. a) Expression (RPKM+.5) of piRNA pathway transcripts between egg chambers of young and old females. Red dots indicate transcripts that were in the top 10% of significant FDR-adjusted p-values. b) Log2 ratios of old to young piRNA pathway transcript expression (RPKM+.5) in egg chambers across strains. Both strains show a that a majority of piRNA transcripts increase with age. Relative to similarly expressed genes, the piRNA machinery shows a significantly higher level of expression in older egg chambers (permutation test, p = 0.0131)