Table 2.

Ecotoxicological tests of 4% TDS against Coturnix japonica, Brachydanio rerio and Macrobrachium nipponense

Species	Test	Duration and conditions	Toxicity
Coturnix japonica, aged 30 days, each weighing approximately 100 g, 5 males and 5 females for each group	Acute oral toxicity, repeated in triplicate	20–25 °C air temperature; 0, 6, 12, 24, 36, 60 mg/kg doses; a single dose by gavage; 7 days	1, 2, 3 and 7 d LD50 were above 60 mg/kg; moderate toxicity (50 < acute oral LD50 ≤ 500 mg/kg)
Brachydanio rerio, about 0.4 g in body weight and 3.5 cm in body length, 10 for each group	Acute toxicity, repeated in triplicate	20 °C water temperature; 0, 0.04, 0.1, 0.15, 0.2, 0.4 mg/L concentrations; semi-static, 96 h	24, 48, 72 and 96 h LC50 were 0.19 (0.16–0.22), 0.13 (0.12–0.14), 0.15 (0.14–0.17) and 0.15 (0.14–0.17) mg/L, respectively; high toxicity (0.1 < 96 h LC50 < 1.0 mg/L)
Adult Macrobrachium nipponense, about 2.0 g, 10 for each group	Acute toxicity, repeated in triplicate	20 °C water temperature; 0, 1, 2, 3, 5, 10 mg/L concentrations; semi-static, 96 h	24, 48, 72 and 96 h LC50 were > 10, 8.41 (5.54–12.8), 7.07 (4.96–10.1) and 6.28 (3.53–11.2) mg/L, respectively; moderate toxicity (1.0 < 96 h LC50 < 10 mg/L)