Figure 1.

Adult Staufen2 knock-down (Stau2^GT) mice show an upregulation of cerebellin1 (Cbln1) and glutamate receptor ionotropic delta subunit 2 (Grid2) mRNA during behavioral tests. (A) Schematic representation of the experimental approach followed by the behavioral tests. Here, 4-month-old Stau2^GT and wildtype (WT) mice were exposed to a battery of behavioral tests (OF = open field test, NOR/NOL = novel object recognition/location test, and BM = Barnes maze), enabling physical trainings (= trained). Subsequently, the mouse brains were dissected, and microarray analysis was performed. (B) Microarray analysis of brain lysates of 5-month-old Stau2^GT and WT mice before and after training conditions. (C,D) Quantification of Cbln1 (C) and Grid2 (D) mRNA levels before (naive) and after behavioral tests (= trained) measured by qRT-PCR. N = 3 animals (B–D). Statistics: Students t-test. Mean + SEM, * p < 0.05, *** p < 0.001; ns = not significant (B–D).