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. 2019 Apr 9;20(7):1741. doi: 10.3390/ijms20071741

Figure 2.

Figure 2

Activation of WNT/β-catenin signaling and of stem cell niche proliferation in human hair follicle growth ex vivo in a defined glycosaminoglycan hydrogel matrix (HC007). (A) Quantitative expression analysis by qRT-PCR analysis of selected genes. Input mRNA was obtained from three or four hair follicles per experimental condition, namely, control and HC007 1.5% samples, and the mean +/- SD of relative gene expression values, normalized to 18S rRNA, is represented. **, significant p ≤ 0.1. (B,C) Confocal microscopy images (maximum projections) of the immunolocalization of the (B) transcriptional target of WNT signaling CCDN1 (in red; white dotted lines delineate individual hair follicles; withe arrowheads indicate positive cells in the outer root sheath) and of the (C) CK15 stem cell marker (white dotted boxes are enlarged in right panels) in histological sections of human hair follicles grown ex vivo four days after HC007 1.5% treatments. DAPI was used as chromatin counterstain. Results shown in (B,C) are representative of at least 10 hair follicles in three independent experiments. Bars: 100 µm.