Fig. 4.

Pearson’s Correlation coefficients (r) values were calculated to verify the biological reproducibility between independent replicates in each group (WT and mutants). Each data point represents a single phosphorylation site that was identified and quantified versus the non-stimulated control sample. The extent to which the relative quantitation of the biological replicates (rep 1, rep 2) are reproducible, can be determined by how closely the replicate values produce a correlation closest to 1. (a) At both early (12.5 min) and (b) the late time (at 90 min) points after G-CSF stimulation, the reproducibility observed between the replicates were all correlated at >0.85. Axis for each replicate are on a log base 2 scale.